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miR-409-3p targets the 3′-untranslated region (3′-UTR) of high-mobility group nucleosome binding domain 5 (HMGN5). (A) Schematic of the miR-409-3p-binding sites in the HMGN5 3′-UTR. (B) Dual-luciferase assays of miR-409-3p and HMGN5 3′-UTR. Wild-type or mutated 3′-UTR of HMGN5 was cloned into luciferase reporter vectors. U251 cells were transfected with miR-409-3p mimics or inhibitor in addition to the luciferase reporter vector and incubated for 48 h. *p < 0.05 versus NC.
