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. 2017 Nov 2;25(9):1607–1616. doi: 10.3727/096504017X14938093512742

Figure 4.

Figure 4

ERK1/2 is involved in TGF-β1-induced HepG2 cell invasion. HepG2 cells were treated with TGF-β1 with or without SB431542 for 24 h. Western blot was then used to examine the protein levels of (A) phospho (p)-Smad2 and total (t)-Smad2, as well as (B) p-ERK1/2 and t-ERK1/2, respectively. GAPDH was used as an internal reference. HepG2 cells were then treated with TGF-β1 with or without U0126 for 24 h. (C) Transwell assay was conducted to examine cell invasion. Western blot was used to examine the protein levels of (D) EMT-related genes and (E) MMP9. GAPDH was used as an internal reference. Nontreated cells were used as the control group. **p < 0.01.