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. 2017 Jul 5;25(6):897–912. doi: 10.3727/096504016X14816352324532

Figure 2.

Figure 2

CFEZO treatments induced morphologic features of apoptosis in HepG2 cells. The cells were treated with the indicated concentrations of CFEZO for 24 h, after which cells were examined for emergence of apoptotic hallmarks. (A) Light microscopy photomicrographs showing morphological changes in HepG2 cells after incubation with CFEZO. Cellular shrinkage, detachment, and irregularity of cell shape are notable [magnification: 20× (I), 40× (II)]. (B) Cells stained with 1 μg/ml AO/EtBr for 15 min at 37°C and visualized by fluorescence microscope [magnification: 20× (I), 60× (II)]. (C) Cells were stained with vital nuclear stain (Hoechst 33342) for 15 min at 37°C and visualized by a fluorescence microscope (magnification: 20×).