Figure 3.

CFEZO induces accumulation of ROS and depletion of GSH in HepG2. (A) Cells were treated with the indicated concentrations of CFEZO for 24 h and then stained with DCFH-DA, and the DCF fluorescence intensity was measured by a fluorescence spectrophotometer. Data are representative results from three independent experiments. (B) Cells were treated with the indicated concentrations of CFEZO for 24 h and then assayed for GSH levels as detailed in the Materials and Methods section. The results are presented as the mean ± SD of three independent experiments. Statistical significance in ROS/GSH levels of *p < 0.05, **p < 0.01, and ***p < 0.001 compared to that of control.