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. 2021 Jan 14;11:626216. doi: 10.3389/fmicb.2020.626216

FIGURE 2.

FIGURE 2

Concentration-dependent intracellular activities of CFZ in MAC-infected BMDMs. BMDMs were infected with (A) M. avium ATCC 700898 or (B) M. intracellulare ATCC 13950 and treated with the indicated dose of CFZ. (C) BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR. After 72 h of cultivation, bacterial CFUs were enumerated by plating serially diluted cell lysates on 7H10-OADC agar plates. Each experiment was repeated at least twice independently with triplicate wells; the results of a representative experiment are shown. Each dot represents the mean value ± S.D. of duplicate or triplicate wells, with four spots applied per well. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. ***p < 0.001 vs. Pre-Tx. Pre-Tx, pre-treatment; CTL, untreated control; hpi, h post-infection.