Table 1.

List of gRNA plasmids in the “PCR & Go” toolkit.

	Vector backbones
	pRS423 (HIS3)	pRS426 (URA3)
Mono-gRNA plasmids	p423-sgRNA-M1 p423-sgRNA-M2 p423-sgRNA-M3 p423-sgRNA-M4 p423-sgRNA-M5 p423-sgRNA-M6 p423-sgRNA-M7 p423-sgRNA-M8	p426-sgRNA-M4 p426-sgRNA-M6 p426-sgRNA-M8
Dual-gRNA plasmids	p423-sgRNA-M1-M2 p423-sgRNA-M2-M5 p423-sgRNA-M4-M5 p423-sgRNA-M4-M8 p423-sgRNA-M5-M8 p423-sgRNA-M7-M8	p426-sgRNA-M4-M5 p426-sgRNA-M4-M6 p423-sgRNA-M4-M8 p426-sgRNA-M5-M8
Triple-gRNA plasmids	p423-sgRNA-M1-M2-M3 p423-sgRNA-M1-M2-M4	p426-sgRNA-M2-M3-M5 p426-sgRNA-M4-M5-M6 p426-sgRNA-M3-M5-M8
Quadruple-gRNA plasmids	p423-sgRNA-M1-M2-M3-M4	Not constructed