FIGURE 1.

PCR speed tests. PCR performance of wild-type Taq pol on the left half of each agarose gel is compared to TaqD732N on the right half. The gap size (distance between 3′-ends of the primer pairs) was 270, 502, 1000 or 1500 as labeled. Thermal cycler machine settings for annealing/extension time in seconds was tested in pairs with the shorter time on the top half of each gel. (A) 2 and 8 s (B) 5 and 20 s. Other timings 1, 10, 15, 18, 25, and 30 s are shown in Supplementary Figure 2.