TABLE 2.

Templates and primers.

Experiment	Template	Left primer(s)	Right primer(s)
Crude lysate PCR screen	None added, except as the crude bacterial extract/enzyme. PCR target was rDNA.	DG74: AGGAG GTGATCCAACCGCA (Teng et al., 2004)	RW01; AACTG GAGGAAGGTGGGGAT
PCR speed test	Human DNA (Novagen) 3.7 ng per 40 μl reaction; CCR5 gene was targeted.	16.136 CCTGACAATC GATAGGTACC TGGCTGT	16.125′ CTTCT CATTT CGACA CCGAA GCAGA (270 bp gap);17.70′ CGTTT CTGAA CTTCT CCCCG ACAAA GGC (502 bp gap);17.71′ TCTGT TCAGA TCACT AAACT CAAGA ATC (1000 bp gap)
LAMP assay for DNA	Phage lambda DNA (New England Biolabs), 1 ng per 25 μl reaction.	14.163 F3 lambda GTAAA AACAC CTCAC GAGTT;14.165 FIP lambda: TCCTA CGGTC AAGAG AAGCAATAAA (-) CACCT AAGTT CTCAC CGAAT	14.164 B3 lambda TTTAC GAACA TTAAG CGACTT;14.166 BIP lambda: CTTTCCACATGC AGGAT TTTGG (-) ATG CACGC AATGG TGTAG
MS2 RT-LAMP	MS2 RNA (Roche) as 1 μl of 2–3 ng/μl in 0.1X reaction buffer. Primer set suggested by Chander et al. (2014).	15.36 MS2F3 TGTCA TGGGA TCCGG ATGTT; 15.38 MS2FL CAGAG AGGAG GTTGCCAA; 15.40 + Mly MS2FIP GCCC AAAC AACGA CGATC GGTA gagtc AAAC CAGCA GTAGCCT;	15.37 MS2B3 CAATA GAGCC GCTCT CAGAG;15.39 MS2BL TGCAG GATGC AGCGCCTTA; 15.41 + Mly MS2BIP GCACGTTC TCCAACG GTGCT gagtc GGTTG TTGT TCAGC GAACT.
MS2 RT PCR	Same as above, but only 5 pg per reaction.	MS2-P-FOR: GTATC TTGAA CCCAC TAGGT ATAG;MS2-K-FOR: AGTTC GGTTG GTTAC CACTAAT	MS2-P-REV: CGACG AGAAC GAACTGAGTAA;MS2-K-REV: AAGGT ATGGA CCATC GAGAAAG