Mineral salt mixture
Salts	Concentration (w/v)	Amount for 1 L medium (μL)
MnCl2·4H2O	2%	40
Na2B4O7·10H2O	2%	105
ZnSO4·7H2O	2%	5.5
CuSO4·5H2O	0.2%	12.5
Na2MoO4·2H2O	0.2%	7.5
VOSO4·5H2O	0.2%	7.5
CoSO4·7H2O	0.2%	2.5
NiSO4·6H2O	0.2%	2.5
FeSO4·7H2O	2%	100
In total		283

Measure each salt in a beaker and add ~80 mL water to resuspend the salts. For FeSO₄, add 0.5 M HCl until the salt is dissolved, supplement water up to 100 mL, and filter-sterilize the solution through a 0.22-μm filter.