| Restriction digestion components | |
|---|---|
| Component | Volume (μL) |
| 10× FastDigest buffer | 5 |
| Plasmid/ PCR product | About 1 μg |
| FastDigest NdeI | 1 |
| FastDigest SalI | 1 |
| H2O | Up to 50 |
| Total | 50 |
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