Table 3.
List of genes to detect in the 3D culture and their expected dCt values (normalized to 18s)
| Gene | Encoded protein | Association | Day | dCt (Target gene – 18s) |
|
|---|---|---|---|---|---|
| Min | Max | ||||
| Dcx | Doublecortin | immature neurons | 1 | 9.57 | 13.97 |
| 14 | 12.13 | 16.15 | |||
| 21 | 13.58 | 18.27 | |||
| Pax6 | Paired Box Protein Pax-6 | immature neurons | 1 | 14.08 | 21.20 |
| 14 | 17.06 | 23.34 | |||
| 21 | 17.01 | 17.83 | |||
| Syn1 | Synapsin I | presynaptic terminal | 1 | 14.39 | 19.00 |
| 14 | 14.05 | 17.57 | |||
| 21 | 12.59 | 17.84 | |||
| Shank3 | SH3/ankyrin domain gene 3 | postsynaptic terminal | 1 | 15.62 | 16.58 |
| 14 | 15.37 | 15.73 | |||
| 21 | 14.04 | 15.28 | |||
| Slc17a7 | Vesicular glutamate transporter 1 | glutamate neurotransmission (presynaptic) | 1 | 16.31 | 19.97 |
| 14 | 13.96 | 16.22 | |||
| 21 | 11.64 | 15.83 | |||
| Gria1 | Glutamate Ionotropic Receptor AMPA Type Subunit 1 | glutamate neurotransmission (postsynaptic) | 1 | 14.28 | 18.44 |
| 14 | 14.32 | 17.13 | |||
| 21 | 13.60 | 17.28 | |||
| Grin1 | Glutamate Ionotropic Receptor NMDA Type Subunit 1 | glutamate neurotransmission (postsynaptic) | 1 | 14.81 | 19.64 |
| 14 | 14.27 | 18.14 | |||
| 21 | 13.16 | 16.40 | |||
| Slc1a3 | Excitatory amino acid transporter 1 | GABA neurotransmission (presynaptic) | 1 | 14.27 | 18.95 |
| 14 | 13.13 | 16.09 | |||
| 21 | 12.12 | 16.90 | |||
| Gabra1 | Gamma-aminobutyric acid type A receptor alpha1 subunit | GABA neurotransmission (postsynaptic) | 1 | 16.67 | 19.16 |
| 14 | 14.62 | 16.46 | |||
| 21 | 12.32 | 15.15 | |||