| Neuro medium | Final concentration | Amount |
|---|---|---|
| Neurobasal medium minus phenol red | n/a | 480 mL |
| B27 supplement | 2% | 10 mL |
| Antibiotic-antimycotic | 1% | 5 mL |
| GlutaMax | 2 mM | 5 mL |
| Total | n/a | 500 mL |
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