FIGURE 3.

Treatment of Sac/Val in rats accompanied with HFpEF significantly alleviated the vascular injury of HFpEF induced by high-salt diet. (A) The primary procedures of evaluating the vascular endothelium diastolic function (n = 10 in each group). (B, C) Acetylcholine (Ach) is used to measure endothelium-dependent vasodilation and sodium nitroprusside (SNP) to measure endothelium-independent vasodilation. (D) The levels of NO in serum of rats were detected to evaluate the vascular injury. (E) H&E and Masson’s trichrome staining were used to assess the pathological changes of blood vessel. (G) Assessment of vascular fibrosis by Masson’s trichrome staining; quantification of fibrotic areas. (F, H, I) Immunohistochemical staining of Collagen 1 and Collagen 3 in blood vessel. Quantification of Collagen 1 and Collagen 3 areas: *p < 0.05 means statistic significance; NS means no statistic significance. All these data represent the mean ± SD of at least three times of the experiment.