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. 2021 Jan 14;11:600953. doi: 10.3389/fphar.2020.600953

FIGURE 4.

FIGURE 4

Treatment of Sac/Val can inhibit cardiac fibrosis via TGF-β1/Smad3 signaling pathway potentially. (A, L) Western blot analysis of protein expression levels in rats heart tissues (n = 10 in each group). The proteins include Collagen 1, Collagen 3, MMP2, TIMP2, Smad 7, TGF-β1, and Smad3. (B–E, G) Statistic analysis of protein expression levels of Collagen 1, Collagen 3, MMP2, TIMP2, and Smad 7. (F) The ratio of MMP2/TIMP2 was calculated. (H–J) qRT-PCR analysis of Collagen 1, Collagen 3, and Smad 7 mRNA expression levels in heart tissues. (K) Immunohistochemical staining of Collagen 1 and Collagen 3 in heart tissues. (M, N) Statistic analysis of protein expression levels of TGF-β1 and Smad3. (O) qRT-PCR analysis of TGF-β1 mRNA expression level in heart tissues. *p < 0.05 means statistic significance; NS means no statistic significance. All these data represent the mean ± SD of at least three times of the experiment.