Table 2.
Primers for qPCR
| Target | Forward primer sequence | Reverse primer sequence | Source |
|---|---|---|---|
| 18S | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | |
| AT-1 (Slc33A1) | GGAATCGTTACCCTTTCAGATTT | GATCCCTTGGGTCTCTTCTTT | Dr Luigi Puglielli |
| ATF4 | CCTATAAAGGCTTGCGGCCA | GTCCGTTACAGCAACACTGC | |
| ATF6 | TCGTGTTCTTCAACTCAGCAC | TGGAGTCAGTCCATGTTCTGT | Dr Feyza Engin |
| Ccl5 | CCTCACCATATGGCTCGGAC | ACGACTGCAAGATTGGAGCA | |
| CHOP | ACCTGAGGAGAGAGTGTTCCA | CAAGGTGAAAGGCAGGGACT | L. Antonucci, J.B. Fagman, J.Y. Kim, J. Todoric, I. Gukovsky, M. Mackey, M.H. Ellisman and M. Karin. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ERstress, PNAS, 112, 2015, 6166-6174. |
| Cyclophilin A | CTGCCAAGACTGAATGGCTG | CCCAAAACGCTCCATGGCTT | |
| XBP1s | CTGAGTCCGAATCAGGTGCAG | GTCCATGGGAAGATGTTCTGG | X. Zhu, F. Yao, Y. Yao, N. Dong, Y. Yu and Z. Sheng. Endoplasmic reticulum stress and its regulator XBP-1 contributes to dendritic cell maturation and activation induced by high mobility group box-1 protein, Int J Biochem Cell Biol, 44, 2012, 1097-1105. |
| XBP1(tot) | TGGCCGGGTCTGCTGAGTCCG | GTCCATGGGAAGATGTTCTGG | Zhu et al, 2012; JBC |