Table 1.
QUAST statistics for genome assemblies of flax cultivar Atlant.
| Feature | Canu 2.0 contigs | Canu 2.0 unitigs | Flye 2.7 | Shasta 0.5.0 | wtdbg2 2.5 |
|---|---|---|---|---|---|
| Total assembly length, Mb | 361.7 | 393.9 | 346.1 | 290.8 | 212.2 |
| Number of contigs | 2458 | 4361 | 8278 | 5641 | 2306 |
| Largest contig, Mb | 5.0 | 1.7 | 3.3 | 2.0 | 3.0 |
| GC, % | 38.94 | 38.93 | 38.98 | 39.06 | 39.03 |
| N50, kb | 350 | 225 | 191 | 295 | 365 |
| NG50, kb | 412 | 286 | 222 | 264 | 117 |
| N75, kb | 154 | 84 | 51 | 142 | 112 |
| NG75, kb | 220 | 159 | 84 | 106 | – |
| L50 | 261 | 472 | 368 | 278 | 141 |
| LG50 | 201 | 319 | 295 | 323 | 397 |
| L75 | 648 | 1191 | 1205 | 634 | 407 |
| LG75 | 463 | 685 | 871 | 789 | – |
Key parameters are marked in bold. NG50/NG75 is the maximum length for which the subset of contigs of that length or longer covers at least 50%/75% of the reference genome (cultivar CDC Bethune, GenBank: GCA_000224295.2). LG50/LG75 is the number of contigs with a length equal to or greater than NG50/NG75, that is, the minimal number of contigs that cover 50%/75% of the reference genome. Unitigs are high-confidence contigs, according to Canu terminology.