Figure 1.

Effects of A) dehydroepiandrosterone (DHEA) (0-40 µ g/ml), B) androstenedione (0-20 µ g/ml), and C) testosterone (0-20 µ g/ml) on surtuin 1 (SIRT1) expression in human aortic endothelial cells at 24 hours after the start of treatment. Data are shown as the mean±SD of the SIRT1/ß-actin densitometric ratio with each agent relative to that with control medium determined in real-time polymerase chain reaction analysis in 2 separate experiments. *p<0.05, **p<0.01, ***p<0.001 versus control.