Table 1. Kinetic Parameters of the Pure Compound on HPA with Starch and Maltopentaose as Substrates.

substratea	DDEB (μM)	KMb	Vmax (μM min–1)	Kcat (min–1)	Kcat/KMc	Ki′ (μM)
starch	control	7056.04 ± 352.80	0.641 ± 0.032	0.115 ± 0.0058	1.63 ± 0.08 × 10–5	2.49
	9	1707.48 ± 85.38	0.154 ± 0.008	0.027 ± 0.0014	1.61 ± 0.08 × 10–5
	18	697.56 ± 34.88	0.066 ± 0.033	0.012 ± 0.0006	1.69 ± 0.08 × 10–5
	27	394.69 ± 19.74	0.038 ± 0.002	0.007 ± 0.00035	1.70 ± 0.09 × 10–5
	36	256.16 ± 12.81	0.025 ± 0.001	0.004 ± 0.0002	1.72 ± 0.09 × 10–5
maltopentaose	control	132.48 ± 6.62	0.040 ± 0.002	0.0071 ± 0.0004	5.39 ± 0.27 × 10–5	47.6
	9	97.98 ± 4.90	0.028 ± 0.001	0.0050 ± 0.0003	5.11 ± 0.26 × 10–5
	18	72.29 ± ± 3.62	0.021 ± 0.001	0.0038 ± 0.0019	5.24 ± 0.26 × 10–5
	36	62.71 ± 3.14	0.018 ± 0.0009	0.0032 ± 0.0002	5.06 ± 0.25 × 10–5
	54	52.21 ± 2.61	0.015 ± 0.0008	0.0027 ± 0.0014	5.22 ± 0.26 × 10–5

^aStarch (1–5 mg mL^–1), maltopentaose (50–400 μM), and 5.59 μM HPA were used for assays.

^bUnit of K_M is μg for starch and μM for maltopentaose.

^cUnit of K_cat/K_M is min^–1 μg^–1 for starch and min^–1 μM^–1 for maltopentaose.