Table 1. Comparison of Kinetic Values of Three Oxidases Obtained in the Fluidigm System and a Microplate Reader.

enzyme	system	KM [mM]a	Vmax [μmol mg–1 min–1]a
lactate oxidase	Microplate	1.62 ± 0.48	22.00 ± 1.15
	Chip	1.23 ± 0.12	23.67 ± 2.73
	Literatureb	0.5–1	114–270
glutamate oxidase	Microplate	0.20 ± 0.01	6.30 ± 0.25
	Chip	0.29 ± 0.08	7.30 ± 1.00
	Literatureb	0.17–0.3	6–55
glucose oxidase	Microplate	20.67 ± 2.72	2.40 ± 0.00
	Chip	16.33 ± 1.76	23.00 ± 4.58
	Literatureb	22–32	6–170

^aValues for the microplate and the chip represent the mean average (±standard error of mean; n = 3).

^bLiterature values ranges, as reported for wild-type enzymes in BRENDA DB.¹²