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. 2021 Jan 28;22:82. doi: 10.1186/s12864-021-07368-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — ASTTI evaluation of two lowland switchgrass genotypes (Alamo and AM-314/MS-155) by the physiological changes in roots under alkaline salt stress for 0, 3, 6, 12 and 24 h. a: Relative water content (RWC). b: Relative electric conductivity (REC).c: Malondialdehyde (MDA) content. d: Proline content. e: Soluble protein content. f: Soluble sugar content. g: Glutathione content (GSH). h: Superoxide dismutase (SOD) activity. i: Peroxidase (POD) activity. j: Catalase (CAT) activity. The left Y-axis represented value of physiological traitors, and the right Y-axis represented value of ASTTI. The assays were repeated three times with three biological replicates. The data, shown as means ± SEs, were subjected to the Student’s t test and one-way ANOVA with Duncan’s multiple range test to determine significant differences. “*” denotes p < 0.05, “**” denotes p < 0.01. The different letters represent statistically significant differences within genotype (α = 0.05)