Figure 4.

Gamma radiation does not influence pKZ1 A11 mouse hybridoma cell response to overgrowth in low radiation environment. (A) Three cultures of pKZ1 A11 mouse hybridoma cells were set-up in quadruplicate and grown at the ISS reference laboratory in Rome (ISS) and at the Gran Sasso underground laboratory (GS) in a conventional incubator (CI) and in a γ-radiation shielded incubator (SI). Cells were counted and split twice a week (after 72 h and after 96 h) for 4 weeks. Cells were counted in triplicate. Values are means ± SD. (B) Cells grown as in (A) were collected after 3 and 4 weeks of cultures (at 96 h splitting time) and analyzed for apoptotic and autophagic markers by western blots. (C) Densitometric analysis of anti-P-p53 (Ser392), anti-PARP1, anti-caspase3 western blots. Values are means of two different experiments ± SD. *P < 0.05, **P < 0.005, ***P < 0.0005.