Fig. 4.
Caspase-1/IL-1β promoted apoptosis and inhibited membrane transport of GluA1 in Aβ1-42-induced hippocampal neurones. Hippocampal neurones were treated with Aβ1-42 oligomer (10 μmol/L) for 24 h, and then treated with IL-1β (10 μg/L) or AC-YVAD-CMK (10 μmol/L) for 24 h. Aβ1-42-treated hippocampal neurones served as control. a The apoptosis of hippocampal neurones was examined by flow cytometry. b ELISA was performed to assess the levels of IL-1β in the hippocampal neurones. c WB was performed to detect the expression of GluA1 in the hippocampal neurones. S: surface; I: intracellular. (One-way ANOVA; *P < 0.05, **P < 0.01 vs. Ctrl group; #P < 0.05, ##P < 0.01 vs. AC-YVAD-CMK group.)