Fig. 5.
Caspase-1/IL-1β inhibited membrane transport of GluA1 by inhibiting the interaction between Stargazin and GluA1. APP/PS1 mice were intraperitoneally injected with AC-YVAD-CMK or DMSO. WT C57/BL6 mice were served as control. a WB was performed to detect the expression of Stargazin in the hippocampal tissues of mice (One-way ANOVA). Hippocampal neurones were treated with Aβ1-42 oligomer (10 μmol/L) for 24 h. Normal hippocampal neurones were served as control. b WB was performed to assess the expression of Stargazin in the hippocampal neuron cells (Student’s t test). c Co-IP assay was performed to verify the interaction between Stargazin and GluA1 in hippocampal neurones. Hippocampal neurones were transfected with pcDNA3.1-Stargazin mutant or pcDNA3.1-NC. Then, the modified hippocampal neurones were treated with Aβ1-42 oligomer (10 μmol/L) combined with AC-YVAD-CMK (10 μmol/L) for 24 h. d WB was performed to detect the expression of GluA1 in the modified hippocampal neurones (Student’s t test). S: surface; I: intracellular. **P < 0.01 vs. WT group; ##P < 0.01 vs. Ctrl group; $$P < 0.01 vs. Aβ1-42 group