Fig. 4.

MTCH2 knockdown impairs the migration of glioma cells in vitro. a, b Representative images of wound healing assays showing that MTCH2 knockdown inhibits the migration of A172 cells (N = 4). The migration of cells into the wound was assessed at 0, 24, 48 and 72 h. Scale bar, 200 μm. c Images and quantifications showing that MTCH2 knockdown reduces the rate of migration of U87 MG cells by Transwell migration assays. d Immunostaining images showing the reduced F-actin fibers by MTCH2 knockdown in A172 cells. Scale bar, 10 μm. e, f Western blots and quantifications showing that MTCH2 knockdown alters cellular signalings relative to cytoskeleton dynamics