Table D.7.
Advantages/disadvantages related to the ease of use and technical resources required for each method of the methods to detect whether a previously frozen fish is commercially presented as ‘superchilled’
| Method category | Method group | Method subgroup | Execution speed | Destructiveness | Laboriousness | Laboratory equipment | Skills needed | Threshold/calibration setting | Technological readiness |
|---|---|---|---|---|---|---|---|---|---|
| Biochemical | Enzymatic | HADH | M | D | L | ST | SI | T | H |
| β‐N‐acetylglucosaminidase | M | D | L | ST | SI | T | H | ||
| α‐glucosidase | M | D | L | ST | SI | T | H | ||
| Acid phosphatase | M | D | L | ST | SI | T | H | ||
| β‐glucuronidase | M | D | L | ST | SI | T | H | ||
| β‐galactosidase | M | D | L | ST | SI | T | H | ||
| Lactate dehydrogenase | M | D | L | ST | SI | T | H | ||
| Citrate synthase | M | D | L | ST | SI | T | H | ||
| Succinate dehydrogenase | M | D | L | ST | SI | T | H | ||
| Protein markers by electrophoresis | Aspartate aminotransferase | S | D | L | ST | SP | NT | H | |
| Parvalbumin | S | D | L | ST | SP | NT | M | ||
| Transgellin | S | D | L | ST | SP | NT | M | ||
| Profile analysis | Volatile profile | S | D | L | AD | SP | T | M | |
| Phospholipid profile | S | D | L | AD | SP | T | M | ||
| Haematological method | Haematocrit value | F | D | NL | ST | SI | NT | H | |
| Haemolysis of red blood cells | F | D | NL | ST | SI | NT | H | ||
| Others | Free calcium concentration | M | D | L | ST | SI | T | H | |
| Nucleotides and related compounds | M | D | L | ST | SI | T | H | ||
| Morphological | Eye lens | Eye lens | F | D | NL | ST | SI | NT | H |
| Histology | Histology | S | D | L | AD | SP | NT | H | |
| Physico‐chemical | Imaging/spectroscopy | Combinations of Ultraviolet–visible/NIR (UV‐VIS/NIR) | F | ND | NL | AD | SP | NT | H |
| (Front‐face) fluorescence spectroscopy | F | ND | NL | AD | SP | NT | L | ||
| Nuclear magnetic resonance (NMR)/MRI | F | ND | NL | AD | SP | NT | L | ||
| Raman spectroscopy | F | ND | NL | AD | SP | NT | L | ||
| Hyperspectral imaging | F | ND | NL | AD | SP | T | H | ||
| Electrical parameters | Electrical and dielectric impedance | F | ND | NL | AD | SP | NT | L | |
| DSC | DSC | F | ND | NL | AD | SI | T | H |
F = fast execution speed (< 0.5 days); M = medium execution speed (< 1 day); S = slow execution speed (> 1 day).
D = destructive method; ND = non‐destructive method.
L = laborious method (sample preparation necessary, many handling operations); NL = non‐laborious method (no or easy sample preparation, few handling operations).
ST = standard laboratory equipment; AD = advanced laboratory equipment.
SI = simple skills needed; SP = specialised skills needed.
T = need of threshold/calibration setting by species or group of species; NT = no need of threshold/calibration setting by species or group of species.
L = low technological readiness for commercial applications; M = medium technological readiness for commercial applications; H = high technological readiness for commercial applications.