Table D.8.

Evaluation table of the biochemical methods to detect whether a previously frozen fish is commercially presented as ‘superchilled’

Method evaluated	Enzymatic									Electrophoresis			Profiles		Haemological methods		Other methods
Evaluation criteria	HADH	\ralpha‐N‐acetyl glucosaminidase	\ralpha‐glucosidase	Acid phosphatase	\rbeta‐glucuronidase	\rbeta‐galactosidase	Lactate dehydrogenase	Citrate synthase	Succinatedehydrogenase	Aspartateaminotransferase	Parvalbumin	Transgelin	Volatile profile	Phopholipid profile	Haematocrit value	Haemolysis ofred blood cells	Free calcium concentration	Nucleotides and related compounds
Applicability of the method to different fish species	++	+	++	0	0	0	0	0	–	+	0	0	++	0	0	+	0	0
Ability of the method to differentiate fresh fish from fish frozen at various temperatures	++	++	++	0	0	0	0	0	–	0	0	0	0	0	+	+	0	0
Use of a single method possible	+++	+	++	+	+	+	–	+	–	+	+	+	++	++	++	+++	++	+
Ease of use of the method	++	++	++	++	++	++	++	++	++	+	+	+	+	+	++	++	++	++
Validation of the method	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Classification performance in discriminating as either frozen/not frozen	++	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Evidence that superchilled fish will behave like fresh fish in the given analysis	+	+	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a	0a
Strength of overall evidence (number of records; average appraisal scoreb)	9; 4.9	7; 3.3	7; 2.9	2; 2.5	1; 1	2; 2	2; 1	1; 5	1; 2	1; 3	1; 3	1; 1	3; 1.7	1; 3	1; 6	1; 4	1; 1	1; 1

0 = no information available; – = poor performance; + = weak performance/weak evidence; ++ = good performance/good evidence; +++ = excellent performance/good evidence.

^aThere is no evidence, but it has been assumed that superchilled fish would behave as fresh fish.

^bThe division of the total appraisal score of all the records and the number of records.