Fig. 2.
Establishment of Proofman-LAMP for SARS-CoV-2 detection. (A) Design of LAMP primer sets and the Proofman probe based on the conservative sequence of SARS-CoV-2 gene N. (B) LAMP assay coupled with or without the Proofman to amplify the pUC57-N plasmid containing SARS-CoV-2 gene N cDNA. (C) Agarose gel image of the LAMP reaction (with/without Proofman probe) products (bottom) and endpoint image under 475 nm blue light (upper). PC, positive reaction with the template; NC, negative control reaction without the template; M, DNA marker. (D) Detection of SARS-CoV-2 gene N RNA using RT-Proofman-LAMP. (E) Endpoint image of RT-Proofman-LAMP after 50 min incubation. The reactions contained 1 × 106 copies of DNA/RNA templates except for negative control (NC). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)