Fig. 5.
Evaluation of RT-Proofman-LAMP using spiked saliva samples. (A) The general workflow to detect saliva samples using Proofman-coupled LAMP. (B) Visual detection of singleplex RT-Proofman-LAMP. The spiked sample contained 50 copies of pseudovirus. (C) Duplex assay of RT-Proofman-LAMP using spiked saliva sample (containing 50 copies of pseudovirus). (D) Triplex assay of RT-Proofman-LAMP using spiked saliva sample (containing 50 copies of pseudovirus).