Figure 3.

hMSCs were stained for αvβ3 cell surface integrin, resulting in a fluorescent green staining, and counterstained with DAPI (blue cell nuclei). The positive αvβ3 cells were counted and normalized to the total cell number for each system and presented in graphical form (A) for days 2 and 11. Acronyms for the peptide sequences are as follows: CRGDSG, short tether; CRGDSC, dually attached; C(G)gRDGSG, spaced tether; CGRDSG, scrambled. (B, C) Micrographs of cells staining for DAPI and αvβ3 on day 11; (B) is that of a pure PEG system and (C) is that of a di-functionalized attached RGD system. *p > 0.01