Figure 1. Construction of the reporter plasmids.

In (A), there is a schematic representation of the vector map of pQC-FSCFP-mCherryFP, which is modified from pQC-XIG vector (from Addgene) with a synthesized nucleotide sequence as shown in (B). The synthesized sequence (5′-strand shown) is to be cloned into the pQC-XIG vector by restriction sites NotI and EcoRV. This cloning will replace the original IRES and GFP regions. It will also introduce the CFP coding sequence and additional cloning sites. In the demonstrated example here it also included the 20 nt target sequence.