Figure 4. A schematic showing flow cytometry gating strategy.

Apply the gating for single and live cells by using forward and side scattering. For the individual fluorescence (GFP and mCherry), gating was applied based on the negative control, GFP-only control, and mCherry-only controls. The gates for selecting GFP and mCherryFP double-positive cells should be at least one fold above the upper edge of the signals from the GFP-only and mCherryFP-only cells.