Table 2. Human PSC growth medium.

Composition	Volume (500 ml)	Final concentration
DMEM/F-12 KO serum replacement NEAA solution (100x;10 mM) L-glutamine/2-mercaptoethanol (100 mM)  Basic FGF-2 (100 μg/ml)	390 ml 100 ml 5 ml 5 ml 10 μl	20% 100 μM 1 mM 4 ng/ml