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. 2020 Jul 5;10(13):e3675. doi: 10.21769/BioProtoc.3675

Table 10. IF9S medium.

Composition   Volume   Stock concentration

IF4S (10x)

ddH2O

PVA

GlutaMax (100x)

NEAA(100x)

Holo-transferrin  

Insulin

50 ml

340 ml

100 ml

5 ml

5 ml

500 μl

1 ml

10 g/L

1x

1x

10.6 mg/L

20 mg/L