Figure 5. Streptavidin-Dylight 650 detection of biotinylated (red) and non-biotinylated (grey) peptides.

For this acquisition, MRBLEs were imaged in the Cy-5 fluorescence channel using a SOLA LED light engine at 5% intensity with a 100 ms exposure time. Images were processed and beads decoded using the MRBLE software package as described previously ( Harink et al., 2019 ). For each code, all Cy5 intensities for each bead were plotted (small markers) along with the median intensity and standard deviation (large markers, error bars) using custom software written in a Python Jupyter notebook.