Table 1. Average yield and viability of cells following digestion and negative selection of ONE fourth mammary fat pad from a wild-type Charles River Sprague-Dawley female.
Postnatal Age (Days) | Average number of cells digested (x 106) | Average viability after dissociation (%) |
Average number of negatively selected cells (x 106) |
8 (n = 1) | 3.0 | 72 | --- |
20-30 (n = 20) | 16.7 | 56 | 4.4 (n = 7) |
30-40 (n = 16) | 33.0 | 44 | 6.6 (n = 3) |
> 40 (n = 26) | 88 | 41 | 2.9 (n = 7) |