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. 2020 Nov 20;10(22):e3818. doi: 10.21769/BioProtoc.3818

Table 1. Average yield and viability of cells following digestion and negative selection of ONE fourth mammary fat pad from a wild-type Charles River Sprague-Dawley female.

Postnatal Age (Days) Average number of cells digested (x 106) Average viability after dissociation (%)

Average number of negatively selected cells

(x 106)

8 (n = 1) 3.0 72 ---
20-30 (n = 20) 16.7 56 4.4 (n = 7)
30-40 (n = 16) 33.0 44 6.6 (n = 3)
> 40 (n = 26) 88 41 2.9 (n = 7)