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. 2020 Jun 20;10(12):e3651. doi: 10.21769/BioProtoc.3651

Table 1. An example of the expected data and analysis.

Representative example of an experiment using a pro-amnesic dose of delta9-tetrahydrocannabinol (THC, 10 mg/kg, i.p., light colour) in CD1 and C57BL/6J mice, or its vehicle (VEH, dark colour) as control, administered after the training session. The exploration time (seconds) in the familiar object (F); the exploration time on the second object in the training session (F’); the exploration time in the novel object (N); the total exploration time (ET = F + F’ or ET = F + N); the percentage of exploration time devoted to each object during the training and the test sessions (% ET = [ F/ET ] x 100; % ET = [F’/ET] x 100; %ET = [N/ET] x 100); the discrimination index is calculated for the test session (DI = [N – F]/[N + F]).

CD1 mice C57BL/6J
Training Test Training Test
Mouse F F’ ET F N ET DI Mouse F F’ ET F N ET DI
1 42 45 87 35 67 102 0.31 1 55 45 100 18 49 67 0.46
2 54 36 90 19 41 60 0.37 2 27 23 50 22 70 92 0.52
3 39 34 73 12 39 51 0.53 3 16 18 34 12 43 55 0.56
4 40 36 76 18 52 70 0.49 4 21 24 45 13 40 53 0.51
5 53 58 111 26 45 71 0.27 5 25 24 49 15 47 62 0.52
6 43 49 92 19 72 91 0.58 6 29 33 62 21 64 85 0.51
7 61 56 117 10 47 57 0.65 7 44 42 86 17 37 54 0.37
Mean 47.4 44.9 92.3 19.9 51.9 71.8 0.46 Mean 31 29.8 60.8 16.9 50 66.9 0.49
%ET 51.4 48.6 - 27.7 72.3 - - %ET 51 49 - 25.2 74.8 - -
Mouse F F’ ET F N ET DI Mouse F F’ ET F N ET DI
1 37 32 69 25 32 57 0.12 1 28 32 60 27 39 66 0.18
2 39 46 85 22 33 55 0.2 2 34 28 62 26 22 48 -0.10
3 30 38 68 27 24 51 -0.06 3 29 45 74 33 34 67 0.01
4 51 52 103 54 59 113 0.04 4 35 28 63 21 23 44 0.04
5 49 50 99 37 45 82 0.1 5 39 46 85 26 31 57 0.09
6 57 42 99 19 16 35 -0.09 6 45 51 96 29 31 60 0.03
7 43 51 94 12 14 26 0.08 7 29 38 67 41 38 79 -0.04
Mean 43.7 44.4 88.1 28 31.9 59.9 0.06 Mean 34.1 38.3 72.4 29 31.1 60.1 0.03
%ET 49.6 50.4 - 46.8 53.2 - - %ET 47.1 52.9 - 48.3 51.7 - -