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. 2020 Jan 20;10(2):e3486. doi: 10.21769/BioProtoc.3486

Figure 4. Example calculations to determine LP CD4 T cell depletion using the LPAC model.

Figure 4.

Line A reflects the total number of viable LPMC determined at the completion of the culture period based on trypan blue exclusion counts for both Mock condition and HIV-1 conditions. Line B reflects the percentage of CD3+CD8- LP T cells as a fraction of viable lymphocytes enumerated using multi-color flow cytometry. The total absolute number of LP CD4 T cells in each condition is then calculated by multiplying A with B. The graph reflects the number of LP CD4 T cells in mock and in HIV conditions with the percent depletion in HIV relative to mock shown, calculated as number in HIV-1 condition/number in mock condition (%).