Table 1. Example calculations using an HIV virus strain with a concentration of 20,000 ng p24 per ml.

	A	B	C	D	E
	LPMC (x 106) per 15 ml conical tube	Total p24 (Column A/2.5 x 500)	Total volume(Column A /2.5*1000 μl)	Volume HIV/mock(Column B/20,000 x Column C)	Volume of culture media(Column C-Column E)
HIV	2.5	500 ng	1000 μl	25 μl	975 μl
Mock	2.5	“500 ng”	1000 μl	25 μl	975 μl