Figure 1.

LDM inhibits the growth of B16-F1 cells by inducing apoptosis. (A) MTT method was used to assay the antiproliferation effects of LDM, which inhibits the proliferation of B16-F1 in a dose- and time-dependent manner. Compared with untreated cells in the same time group, *p < 0.05, **p < 0.01. (B) LDM induces B16-F1 cell cycle arrest and stimulate apoptosis detected by flow cytometry. (a) B16-F1 control cells; (b, c, d) B16-F1 cells were treated with concentrations of 2.5 ng/ml, 5 ng/ml, and 10 ng/ml LDM. The arrow indicates the position of sub-G₁. (C) Flow cytometry was performed to show apoptosis of B16-F1 cells treated with 5 ng/ml LDM by Annexin V/PI assay. (D) Western blot assay was used to determine the level of Cyt-C in the cytosol of apoptosis B16-F1 cells treated with LDM.