Figure 2.

Treatment with LDM could enhance phagocytosis of apoptosis B16-F1 cells by inducing membrane translocation of CRT on the cell surface. (A) LDM-induced membrane translocation of CRT on the cell surface of B16-F1 cells was observed by flow cytometry. Cells were incubated with rabbit anti-CRT polyclonal antibody, followed by incubation with rhodamine-conjugated polyclonal antibody. (B) LDM treatment enhanced the phagocytosis of B16-F1 apoptotic cells by DCs or macrophages (MØ) in vitro. (a) Labeled target and effector cells with green dye CFDA-SE and PE-CD11c, respectively. (b) The percentage of macrophages and DCs that have taken up target cells.