Table 2. HPLC gradient used in the lipidomic analysis .

Time (min)	Solvent A (%)	Solvent B (%)	Flow (ml/min)
0	90	10	0.4
2.7	55	45	0.4
2.8	47	53	0.4
9	35	65	0.4
9.1	11	89	0.4
11	8	92	0.4
11.1	0	100	0.4
11.9	0	100	0.4
12	90	10	0.4
12.9	90	10	0.4
13	90	10	0.4
14	90	10	0.4
14.1	90	10	0.4
15	90	10	0.4