Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The first few tubes will likely contain only a low concentration of protein, the middle tubes should contain the bulk of the protein, and the last tubes should contain little or no protein. This indicates that the fluorescent fusion protein has been eluted from the column and shows that the fluorescent protein is properly folded.
