| Step | Problem | Possible solution(s) |
| 7 | No SigP in GOI transcript | Use the hamster IL2 SigP as described in step 7. See vector pAF92 for additional details (https://www.addgene.org/135929/). |
| 13 | GOI segments not in optimal GC% range | Order more than one possible primer to find a combination that can amplify the target sequence with the necessary vector overlap segments. |
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