Table 1. Amount of transfection reagent and retroviral construct recommended per 1x 150 mm plate of 293T cells (scale up as needed).

Tube #1	Amount per 150 mm plate
jetPEI transfection reagent	50 µl
NaCl	450 µl
Total Volume/plate	500 µl
Tube #2	Amount per 150 mm plate
pMSCV-NOTCH1-L1601P-ΔP retroviral construct	20 µg
pCL-Eco packaging construct	15 µg
NaCl	X µl
Fill to Total Volume/plate	500 µl