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. 2016 Jan 21;23(1-2):61–68. doi: 10.3727/096504015X14478843952861

Figure 2.

Figure 2

Overexpressed PDCD4 suppressed cell growth, proliferation, and cell cycle transition. (A) Restoration of PDCD4 protein expression was examined in polyclonal and scramble cells by Western blot. Because of the fusion protein from PDCD4 and green fluorescent protein (GFP), the fusion protein of scramble cannot be detected. β-Actin served as the internal control. (B) MTT cell viability assay was performed on PD4-OE and scramble cells of PC9 and A549. (C) Edu proliferation assay of PD4-OE and scramble cells of PC9 and A549. (D) Colony formation assay was performed on PD4-OE and scramble cells of PC9 and A549. (E) Cell cycle analysis of PD4-OE and scramble cells of PC9 and A549. Data were presented as mean ± SD for three independent experiments.