Figure 4.

HCCR-1 overexpression blocked calcitriol-induced p21 expression and promoter activation. HeLa S3 cells were treated with 0.1% alcohol (control) or 200 nM calcitriol, transfected with pcDNA3.1 or HCCR-1-pcDNA3.1 as indicated, and cultured for 72 h. (A) Western blot analysis demonstrated that calcitriol-induced p21 protein expression was blocked by HCCR-1 overexpression. (B) Real-time PCR showed that calcitriol-induced p21 mRNA upregulation was blocked by HCCR-1 overexpression. (C) Dual-luciferase assay showed that calcitriol-induced p21 promoter activation was blocked by HCCR-1 overexpression.