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SMAR1 was successfully overexpressed in human breast cancer cell line MCF7. Fluorescence microscope (A) and flow cytometry analysis (B) showed that the detection rate of GFP+ cells in both MCF7-c group and MCF7-SMAR1 group were higher than 85%. Western blot analysis (C) displayed that SMAR1 was successfully overexpressed only in MCF7-SMAR1 group.
