Table 2. In vitro PKA kinase assay data analysis.

Schematic view of steps A-H for data processing.

		1/10 Kinase buffer control	Negative control	Positive control	Test sample 1	Test sample n
		A	B	C	D	E
CPM read 1	1	A1	B1	C1	D1	E1
CPM read 2 (duplicate)	2	A2	B2	C2	D2	E2
CPM read 3 (triplicate)	3	A3	B3	C3	D3	E3
Mean	4	(A1+A2+A3)/3	(B1+B2+B3)/3	(C1+C2+C3)/3	(D1+D2+D3)/3	(E1+E2+E3)/3
Corrected mean	5	-	-	C4-B4	D4-B4	E4-B4
Dilution factor (df) = Total volume in reaction tube / Volume spotted on P81
Total CPM in tube	6	A4 x 10	-	C5 x df	D5 x df	E5 x df
CPM of A6 are representative of the ρmols of ATP (“a”) added to the reaction medium
Mixtures contains "b" CPM/ρmol ATP, equivalent to (A4/"a")/1000
Incorporated ATP during the reaction (ρmol)	7	-	-	C6/"b"	D6/"b"	E6/"b
Incorporated ATP in the reaction per min (ρmol/min)	8	-	-	C6/30	D6/30	E6/30