Table 3. Example of results.
The table exemplifies results obtained using the PKA inhibitor H-89 (Sigma #B1427, diluted in DMSO), at the concentrations specified. Note: In this example, all tubes contained cAMP and IBMX (see Table 1) .
| 1/10 Kinase buffer control | Negative Control | H89 (μM) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 (positive control) | 0.1 | 1 | 10 | 50 | 100 | ||||
| CPM reads | replicate 1 | 275374 | 10172 | 303106 | 321092 | 239832 | 44063 | 12470 | 11207 |
| replicate 2 | 285443 | 11604 | 333105 | 327384 | 209389 | 48771 | 10125 | 12164 | |
| replicate 3 | 239039 | 10563 | 313784 | 32639 | 219980 | 43795 | 12634 | 10993 | |
| mean | 266618.67 | 10779.67 | 316665.00 | 227038.33 | 223067.00 | 45543.00 | 11743.00 | 11454.67 | |
| mean - negative control | 0.67 | 305886.00 | 216259.33 | 212288.00 | 34764.00 | 964.00 | 675.67 | ||
| total CPM (10 μl of 40 μl) | 1223544.00 | 865037.33 | 849152.00 | 139056.00 | 3856.00 | 2702.67 | |||
| 800 pmoles of ATP represents 2666190 CPM | |||||||||
| Thus, mixtures contains 3332.7 CPM/pmol ATP | |||||||||
| incorporated pmol of ATP in the reaction (pmol) | 367.13 | 259.56 | 254.79 | 41.72 | 1.16 | 0.81 | |||
| incorporated pmol of ATP in the reaction per min (pmol/min) | 12.24 | 8.65 | 8.49 | 1.39 | 0.04 | 0.03 | |||
| Since each tube contains 2.40E5 sperm, then incorporated ATP in the reaction (pmol/min/5E6sperm) | 254.91 | 180.22 | 176.91 | 28.97 | 0.80 | 0.56 | |||