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. 2020 Jul 5;10(13):e3676. doi: 10.21769/BioProtoc.3676

Figure 1. Cartoon representation and example result of the four steps of the binding experiment.

Figure 1.

(1) Activation of the NTA chip with nickel. (2) Binding of the his-tagged protein ligand to the chip. (3) Binding of the protein analyte. (4) Regeneration of the chip with EDTA to remove everything bound to start the cycle again. Steps 1 and 2 are only carried out on Flow cell 2 whereas steps 3 and 4 are carried out over both flow cells. The sensorgram shown represent a typical trace of the subtracted FC2-1 sensorgram.